Kinetic Study on Stability of Schiff Base of Pyridoxal 5′-Phosphate and Leucine in Water Media with Cationic Surfactants
Miguel A. Vázquez
Dpt. de Química, Facultad de Ciencias, Universidad de las Islas Baleares, E–07071 Palma de Mallorca
Search for more papers by this authorFrancisco Muñoz
Dpt. de Química, Facultad de Ciencias, Universidad de las Islas Baleares, E–07071 Palma de Mallorca
Search for more papers by this authorCorresponding Author
Josefa Donoso
Dpt. de Química, Facultad de Ciencias, Universidad de las Islas Baleares, E–07071 Palma de Mallorca
Dpt. de Química, Facultad de Ciencias, Universidad de las Islas Baleares, E–07071 Palma de MallorcaSearch for more papers by this authorFrancisco García-Blanco
Dpt. de Química Física, Facultad de Farmacia, Universidad Complutense, E–28040 Madrid
Search for more papers by this authorMiguel A. Vázquez
Dpt. de Química, Facultad de Ciencias, Universidad de las Islas Baleares, E–07071 Palma de Mallorca
Search for more papers by this authorFrancisco Muñoz
Dpt. de Química, Facultad de Ciencias, Universidad de las Islas Baleares, E–07071 Palma de Mallorca
Search for more papers by this authorCorresponding Author
Josefa Donoso
Dpt. de Química, Facultad de Ciencias, Universidad de las Islas Baleares, E–07071 Palma de Mallorca
Dpt. de Química, Facultad de Ciencias, Universidad de las Islas Baleares, E–07071 Palma de MallorcaSearch for more papers by this authorFrancisco García-Blanco
Dpt. de Química Física, Facultad de Farmacia, Universidad Complutense, E–28040 Madrid
Search for more papers by this authorAbstract
We studied the stability of the Schiff bases formed between pyridoxal 5′-phosphate (PLP) and leucine in the presence of (hexadecyl)trimethylammonium bromide (CTAB) over a wide pH range by determining the kinetic constants of formation and hydrolysis of these compounds. The results show that the stability of the Schiff bases is increased by the presence of CTAB as a result of increased rates of formation and decreased hydrolysis rate constants. The ionic head groups of CTAB favour the formation of the bases, while its hydrophobic rests protect the imine double bond from hydrolysis. This model system permits one to obtain partially hydrophobic media with no need for any non-aqueous solvents.
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