The Strange Case of Dr. Jekyll and Mr. Hyde has been Referenced by...

• Batman: The villain Two-Face was inspired by the Jekyll-and-Hyde story; the noble district attorney Harvey Dent has a psychotic break when half his face is scarred by a criminal, leading him to flip a coin to decide whether he's going to embrace his decent or darker impulses. His debut story even featured him reading the story on the first page.
• Marvel Universe: Calvin Zabo, a Mad Scientist, is an in-universe fan of the book, made his own serum based on the one in the book, and even took up the name "Mr. Hyde" as a super villain alias. Unlike Henry Jekyll, though, who had a degree of shame, Zabo as himself is also a pretty horrible person.

Films — Animation

• Doraemon: Nobita and The Space Heroes; There's a villain named Hyde who has a Meaningful Name. Like the novel's character, Hyde appears to be a reasonable and important figure of authority on the surface but is secretly evil on the inside and intends to eliminate an alien planet for his own benefit.

Literature

• The Deadly Experiments of Dr. Eeek: Jekyll and Hyde are mentioned early in the adventure; fittingly enough the book's main villain is another Mad Scientist, not unlike Jekyll.
• In the 1988 miniseries Jack the Ripper, one of the suspects for the Ripper murders is an actor who plays the protagonist on stage. Chief Inspector Abberline also mentions the play when consulting Dr. Gull, who rubbishes the idea of a potion but agrees that someone could be "both sane and insane", foreshadowing for The Reveal that the killer is suffering from multiple personality disorder.
• Welkin Weasels: Professor Speckle Jyde is a mild-mannered gerbil who yearns to be a troublemaker and so creates a potion which turns him into the obnoxious shrew Dr. Lycan Heck.

Radio

• The Navy Lark: In "The Mysterious Radio Signals", Sub-Lieutenant Phillips poorly recalls having seen a late-night film featuring characters called "Dr. Jekyll" and "Mr. Hyde Park":
  Sub-Lieutenant Phillips: I-It doesn't matter, there w-there was only one of them, anyway, an' he was somebody else. Every time he drank his hot fizzy lemonade, he grew a beard, long teeth, and lead boots.

Western Animation

• Inspector Willoughby: In "Hyde and Sneak", the Inspector is on the trail of the famous jewel thief Vampira Hyde. However, every time he catches her, she manages to fool him by taking her magic pills and transforming into a sweet old lady.
• Hare Remover: Elmer Fudd tries to make a potion to turn Bugs into a "deviwish fiend". References the 1941 film version when, upon watching Elmer's reaction to the potion, Bugs tells the audience "I think Spencer Tracy did it better, don't you folks?"