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In Vivo Mesoscopic Microscopy

RUSH3D

High-speed 3D imaging
5 Hz
In vivo digital adaptive optics
400 nm (lateral) 1000 nm (axial)
Resolution
14300 x 10700 x 100
Centimeter-scale field of view
Max 7.0 x 5.4 x 0.40 mm³
Hehu technology enhances imaging performance
RUSH3D achieves ultra-fine perception of 4D light field by deeply integrating technologies such as digital adaptive optics, computational optical sectioning for background removal, and autofocus with scanning light-field sensors, creating a multiple order of magnitude increase in data throughput. In addition, by effectively correcting system and environmental aberrations while maintaining a constant data acquisition rate, we achieved large FOV, high SNR and high-resolution imaging in vivo. RUSH3D uses an integrated system design, providing a user-friendly, compact, and powerful large-field, high-resolution 3D observations to reveal the mysteries of the microscopic world within living organisms.
High usability
Application range
Neuroscience, developmental biology, oncology, immunobiology, pathology, reproductive medicine, tissue engineering and organoids, pharmacodynamic evaluation.
Model animals
Various tissues or organs such as the brain, spinal cord, heart, liver, spleen, lung, kidney, stomach, intestine, testis, ovary, lymph, placenta, and bones in rats and mice, as well as heterogeneous tissues or individuals such as organoids, tumors, zebrafish, newts and Drosophila.
Clear and intuitive software interface
Acquisition
Automatically set and load default experimental settings; acquire multi-dimensional spatial-angular images.
Browsing
Real-time rendering of images being captured or images that have been captured.
Image reconstruction
Powerful analysis functions for one-click batch processing; AI-based motion correction, denoising, and reconstruction.

Specifications

ParametersModelRUSH3D
In Vivo Mesoscopic Microscopy
Objective9.3X/1.05 NA3.1X/0.50 NA
Digital adaptive opticsSupports the correction of aberrations due to spatial heterogeneity of the sample and the system.
Lateral resolution400 nm820 nm
Axial resolution1000 nm9500 nm
Field of view2.6 x 2.0 x 0.05 mm³7.0 × 5.4 × 0.40 mm³
3D acquisition speed5 Hz
Data throughput> 50 GB/s
MultichannelSupports multi-channel acquisition, including four excitation channels at 405/488/561/647 nm, and also allows customization for different wavelengths.
Sample adapter Supports adaptation to different types of sample holders (including but not limited to 35-mm petri dishes, standard glass slides, customized mouse imaging system).
Cell cultureOptional accessories include live cell imaging system and live animal imaging station.

Applications

Brain Science

RUSH3D integrates technologies such as scanning light-field framework, digital adaptive optics, background suppression and autofocus, enabling continuous high-speed observation of neuronal activities in different brain regions. This ensures that biologists can accurately record neural activities at all times, reducing data deviation on the temporal scale.

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High-throughput cell screening

The RUSH3D is capable of rapidly recording morphological changes, locomotion states and interaction processes of millions of cells within millimeter-scale field of view. It significantly enhances the SNR while retaining high spatiotemporal resolution, achieving high-resolution imaging of cells and organelles across a large field of view. THereofore, RUSH3D promotes the development of cell biology.

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Pathology& Disease

The advantages of RUSH3D, such as large field of view, low phototoxicity, and high resolution, can ensure effective observation of the immune responses of cells in different animals under various trauma models, helping researchers explore the intrinsic differences of cells under healthy and disease conditions.

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