"We have sequencing from a colon [tumor] biopsy from a patient who was 4 times vaccinated...we can find [DNA] plasmids in there a hundred copies per cell. They're not exactly the same as Pfizer's, which is a real head-scratcher, but they're in there."
Kevin McKernan (), Chief Scientific Officer and Founder of Medicinal Genomics, as well as former R&D lead of the Human Genome Project, describes for Nick Jikomes () how he and his colleagues have sequenced a colon tumor biopsy from a person who took four COVID injections and have found DNA plasmids—almost certainly from Pfizer's COVID-injection manufacturing process—at a ratio of 100 per cell. McKernan notes that the plasmids, however, are "not exactly the same as Pfizer's," which "is a real head-scratcher."
"We haven't published the work yet, but we have sequencing from a colon biopsy from a patient who was 4 times vaccinated. A year after vaccination, they had a colon cancer. They biopsied it that day, and then 30 days later, they died, and then they biopsied after, and we have sequencing on both the pre-mortem and post-mortem samples," McKernan says. The scientist and entrepreneur, often cited as the first person to find DNA contamination in the mRNA COVID injections, adds, "we can find plasmids in there a hundred copies per cell. They're not exactly the same as Pfizer's, which is a real head-scratcher, but they're in there."
"There's two of them," McKernan says of the plasmids. "And one encodes spike and one encodes nucleocapsid. We don't know where the hell the nucleocapsid one's coming from." (For reference, a nucleocapsid of a virus is the protein shell or capsid that encloses the nucleic acid—DNA or RNA—content, providing protection and structure to the viral genome.)
When asked why the plasmids wouldn't exactly match those used in Pfizer's mRNA-injection manufacturing process, McKernan speculates: "Do they have more than one [DNA plasmid] in circulation? Like, BioNTech got a different manufacturing plasmid than the manufacturing plant out here in the US because they're making these in two different locations? It's possible. Is there contamination in their laboratory, in the manufacturing of this, they get the wrong plasmid in their E. coli vat, and suddenly they've got a different background there?"
"And we've gotta do everything on our end to make sure we didn't introduce it, which we're doing. We're running all types of experiments to show that there's
spike expression going on. But there's any number of reasons that could explain this," McKernan adds.
Note that DNA plasmids—small, circular, double-stranded DNA molecules capable of replicating independently within a bacterial cell—were understood to be used in the manufacturing of the mRNA injections, but should've been removed below a certain threshold established by regulatory authorities such as the FDA. (The DNA contamination may be present in vials at 1,000 times higher than allowable according to one article published by the Brownstone Institute covering this issue. Article title: "The Vax-Gene Files: An Accidental Discovery")
"We've got a case now that we're zeroing in on that looked like the SV40 poly-A signal, which is a termination signal. It's a transcription termination signal.
We've got a piece of that integrating into chromosome 21, and it's breaking a gene that's involved in cancer.
So, that one looks really interesting," McKernan tells Jikomes. "That could be maybe the driver of this whole thing. But the program spits out a long list of potential integrations that we have to go through and verify which ones are real and which ones are artifacts and all that. So I don't wanna get ahead of ourselves on that. That hasn't been Sanger verified yet."
(For reference, Sanger sequencing is a method used to determine the precise order of nucleotides in a DNA molecule; the SV40 promoter is a strong regulatory region from the Simian Virus 40 that drives high levels of gene expression in mammalian cells when used in genetic constructs; a poly-A signal is a sequence in DNA or RNA that signals the end of a gene, prompting the addition of a polyadenine tail to the mRNA during processing, which stabilizes the mRNA and aids in its export from the nucleus.)
"But the copy number alone suggests that these things aren't fully fragmented. Right? These plasmids really shouldn't be replicating to a hundred copies per cell," McKernan adds. "They couldn't, they shouldn't be in there at that level, because if you just do the math on how much is in the vaccine, when you do an injection of this, this person has four vaccines...1.2 ml of Pfizer...went into about 87,000 mls [of] body volume. So you should have a massive dilution into your body. Yet when we're sequencing this and doing qPCR off the tumor, the CTs coming back off the tumor are almost as high as they are straight out of the vial." (For reference, qPCR—or quantitative Polymerase Chain Reaction—is a technique used to amplify and simultaneously quantify a targeted DNA molecule. "CTs" is a reference to cycle threshold; PCR cycle threshold is the number of cycles at which the fluorescence signal from the reaction exceeds the background level, indicating that the target DNA amplification has occurred.)
McKernan goes on to say:
"And even if it were an integration event [that is, the DNA plasmid integrated into the genome]...I think there could be two things going on here: There could be plasmids replicating episomally [in the cell] and there could be parts of them integrated. But if it were purely integrated and the plasmid was gone, we would not expect to see the copy number of what integrated to be higher than the copy number of the genome. Right? You'd get one integration into one chromosome probably. So it would be half the signal of what you get amplifying a human house gene like RNAP, which is what we use. You would get a similar CT if it integrated. Because if it were a driver mutation, the cells would take off, and it would maybe have one copy of that mutation with it. And as a tumor advanced, you would probably you expect to see a CT score in PCR for that region that was similar to the actual genome background. But we're not seeing that. We're seeing CTs that are that are way ahead. If it's a hundred fold up there, it's around six to seven CTs ahead of the RNAP gene, which is the human gene. And then when we do sequencing, we see the same thing.
"The coverage of sequencing is like 100 to 200X in the plasmids [vs.] 1X of the human genome. So they're in this tumor at really high levels. And that tells us that it has to be replicating. And this was a formalin fixed tissue. So it's not like we could sprinkle plasmids on it from our laboratory to contaminate that and have them be translationally active. Formalin is like this process when you take a tissue and you Formalin fix it. It's like...carbon freezing Han Solo. Alright? So you can't add plasmids after the fact and get it to replicate on cells, and you can't add plasmids on the fact afterwards and get it to integrate. Like those things can only occur if the cells are live. So we're pretty certain we've ruled out that, alright, this isn't coming from us. The anti-vaxxers aren't pouring plasmids on this to create a story.
"This has certain biological signals that show this was present in the patient when they were alive. We don't know the source of it. They were four times vaccinated,
and one of the vaccines that they used was one of the earliest vaccines from December 30th 2020."
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