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Plant In Vitro Culture Factories for Pentacyclic Triterpenoid Production

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Plants as Factories for Bioproduction

Part of the book series: Advances in Biochemical Engineering/Biotechnology ((ABE,volume 188))

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Abstract

Pentacyclic triterpenoids are a diverse subclass of naturally occurring terpenes with various biological activities and applications. These compounds are broadly distributed in natural plant resources, but their low abundance and the slow growth cycle of plants pose challenges to their extraction and production. The biosynthesis of pentacyclic triterpenoids occurs through two main pathways, the mevalonic acid (MVA) pathway and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway, which involve several enzymes and modifications. Plant in vitro cultures, including elicited and hairy root cultures, have emerged as an effective and sustainable system for pentacyclic triterpenoid production, circumventing the limitations associated with natural plant resources. Bioreactor systems and controlling key parameters, such as media composition, temperature, light quality, and elicitor treatments, have been optimized to enhance the production and characterization of specific pentacyclic triterpenoids. These systems offer a promising bioprocessing tool for producing pentacyclic triterpenoids characterized by a low carbon footprint and a sustainable source of these compounds for various industrial applications.

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Abbreviations

2 iP:

6-(γ,γ-dimethylallylamino)purine

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

6-Benzyladenine

BeA:

Betulinic acid

CMC:

Cambial meristematic cells

HR:

Hairy roots

JA:

Jasmonic acid and its methyl ester

LS:

Linsmaier and Skoog medium

MeJA:

Methyl jasmonate

MS:

Murashige and Skoog medium

NAA:

Naphthaleneacetic acid

OA:

Oleanolic acid

UA:

Ursolic acid

Wdbr:

Wave-mixed disposable bioreactors

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Authors and Affiliations

  1. Agrobioinstitute, Agricultural Academy, Sofia, Bulgaria

    Ilian Badjakov & Ivayla Dincheva

  2. Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies-Plovdiv, Plovdiv, Bulgaria

    Radka Vrancheva & Atanas Pavlov

  3. Laboratory of Applied Biotechnologies, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Plovdiv, Bulgaria

    Vasil Georgiev & Atanas Pavlov

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  1. Ilian Badjakov
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  2. Ivayla Dincheva
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  3. Radka Vrancheva
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  4. Vasil Georgiev
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  5. Atanas Pavlov
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  1. Institute of Natural Materials Technology, Technische Universität Dresden, Dresden, Germany

    Juliane Steingroewer

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Badjakov, I., Dincheva, I., Vrancheva, R., Georgiev, V., Pavlov, A. (2024). Plant In Vitro Culture Factories for Pentacyclic Triterpenoid Production. In: Steingroewer, J. (eds) Plants as Factories for Bioproduction. Advances in Biochemical Engineering/Biotechnology, vol 188. Springer, Cham. https://doi.org/10.1007/10_2023_245

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