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Electrochemical biosensors based on nucleic acid aptamers

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Abstract

During recent decades, nucleic acid aptamers have emerged as powerful biological recognition elements for electrochemical affinity biosensors. These bioreceptors emulate or improve on antibody-based biosensors because of their excellent characteristics as bioreceptors, including limitless selection capacity for a large variety of analytes, easy and cost-effective production, high stability and reproducibility, simple chemical modification, stable and oriented immobilization on electrode surfaces, enhanced target affinity and selectivity, and possibility to design them in target-sensitive 3D folded structures. This review provides an overview of the state of the art of electrochemical aptasensor technology, focusing on novel aptamer-based electroanalytical assay configurations and providing examples to illustrate the different possibilities. Future prospects for this technology are also discussed.

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Acknowledgment

Financial support from the Spanish Ministry of Economy and Competitiveness (projects CTQ2014-58989-P, CTQ2015-71936-REDT, and CTQ2017-87954-P) is gratefully acknowledged.

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  1. Nanosensors and Nanomachines Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 28040, Madrid, Spain

    Anabel Villalonga, Ana María Pérez-Calabuig & Reynaldo Villalonga

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  1. Anabel Villalonga
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Villalonga, A., Pérez-Calabuig, A.M. & Villalonga, R. Electrochemical biosensors based on nucleic acid aptamers. Anal Bioanal Chem 412, 55–72 (2020). https://doi.org/10.1007/s00216-019-02226-x

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