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Novel fungal organophosphorus hydrolases in acidic media: an application to apples decontamination

Environmental Science and Pollution Research volume 30, pages 10803–10811 (2023)Cite this article

Abstract

Organophosphorus pesticides bring significant improvements in agriculture, but their toxicity causes environmental and health negative impacts. The aim of this work was the development of robust biocatalysts to be applied in bioremediation. Four fungi were evaluated as hydrolase sources capable of degrading organophosphorus pesticides: Aspergillus niger, Fusarium sp., Penicillium chrysogenum, and Penicillium nalgiovense. The hydrolysis rates of methyl paraoxon obtained under acidic conditions were in the range of 10 to 21 mg L−1 d−1, which is remarkable since most similar biocatalysts are active under alkaline conditions. Penicillium chrysogenum activity was outstanding, and it was selected to prepare, characterize, and study the applications of its enzymatic extract. It was used to evaluate the bioremediation of apple surfaces at pH 2 in the presence of SDS, achieving complete methyl paraoxon degradation under proposed conditions. These results indicate that this biocatalyst could complement industrialized fruit washing processes for the elimination of organophosphorus pesticides.

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Data availability

The data sets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

The authors acknowledge Professor Vanesa Ludemann from the Food Mycology Laboratory, Quilmes National University, for kindly providing the fungal strains used in this investigation.

Funding

This study was supported by grants from Universidad Nacional de Quilmes and ANPCyT (PICT 2016–0861). NLR, ESL, and AMI are members of the Scientific Researcher Career of CONICET. JYS is a CONICET fellow.

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Authors and Affiliations

  1. Laboratorio de Biotransformaciones y Química de Ácidos Nucleicos, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, CONICET, Roque Sáenz Peña 352, Bernal (1876), Argentina

    Julia Yamila Santillan, Elizabeth Sandra Lewkowicz & Adolfo Marcelo Iribarren

  2. Laboratorio de Ingeniería Genética y Biología Celular y Molecular-Área Virosis de Insectos, Instituto de Microbiología Básica y Aplicada, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, CONICET, Roque Sáenz Peña 352, Bernal (1876), Argentina

    Julia Yamila Santillan & Natalia Lorena Rojas

Authors
  1. Julia Yamila Santillan
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  2. Natalia Lorena Rojas
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  3. Elizabeth Sandra Lewkowicz
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  4. Adolfo Marcelo Iribarren
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Contributions

All authors contributed to the conception and design of this work. The preparation of the material, data collection, and analysis were in charge of Julia Yamila Santillan and Natalia Lorena Rojas. The manuscript was written by Julia Yamila Santillan and Natalia Lorena Rojas, and all the authors reviewed and modified it. All authors read and approved the final manuscript.

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Correspondence to Julia Yamila Santillan.

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The authors declare no competing interests.

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Responsible Editor: Ta Yeong Wu

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Santillan, J.Y., Rojas, N.L., Lewkowicz, E.S. et al. Novel fungal organophosphorus hydrolases in acidic media: an application to apples decontamination. Environ Sci Pollut Res 30, 10803–10811 (2023). https://doi.org/10.1007/s11356-022-22854-8

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  • DOI: https://doi.org/10.1007/s11356-022-22854-8

Keywords

  • Organophosphorus compounds
  • Methyl paraoxon
  • Fungi
  • Biocatalysts
  • Vegetable and fruit detoxification
  • Enzyme extract
  • Bioremediation