MINI2P - The Kavli Institute for Systems Neuroscience
Mini2P - the brain explorer!
MINI2P is an open-source miniature 2-photon microscope brain explorer for fast high-resolution calcium imaging in freely-moving mice.
Say hello to Mini2P
In brief
Development of a miniature 2-photon miniscope for large-scale calcium imaging in freely moving mice allows stable simultaneous recording of more than a thousand cells across multiple planes of densely active cortical regions in a wide spectrum of behavioral tasks without impediment of the animal's behavior.
Highlights
- We made a light-weight 2-photon miniscope for calcium imaging in freely moving mice
- Stable high-quality imaging was observed during a wide spectrum of behaviours
- Activity can be monitored in volumes of over 1,000 visual or entorhinal-cortex cells
- A custom-designed z-scanning module allows fast imaging across multiple planes
Graphical abstract
Connect with Mini2P
Created by Weijian Zong and maintained by the Moser group at Kavli Institute for Systems Neuroscience. If you have any questions or suggestions, or find any bugs in the codes, please contact us.
Open source on GitHub
MINI2P is a complete open-source project, we encourage people to use, test, modify and further develop this toolbox.
MINI2P_toolbox on GitHub includes the codes, softwares, 3D models, protocols, and etc. for building and using MINI2P to do freely-moving recording.
Paper in Cell
Zong, et al.,"Large-scale two-photon calcium imaging in freely moving mice" Cell (2022).
