抗thyroglobulin抗体の抗idiotype抗体の作成 Induction of idiotypic antiserum against anti-thyroglobulin antibody

In the present paper, we reported the induction of idiotypic antiserum against anti-thyroglobulin antibody from the serum of a patient with chronic thyroiditis. Antisera were raised against IgG F (ab')₂ of anti-thyroglobulin antibodies obtained from 5 patients with chronic thyroditis. After adequate absorptions, only one antiserum was rendered idiotype-specific, as assayed by solid phase radioimmunoassay.<br>Specificity of the idiotypic antiserum was examined by the following ways.<br>1) The idiotypic antiserum reacted with F (ab')₂ of anti-thyroglobulin antibody from patient NAG, but neither F (ab')₂ without anti-thyroglobulin antibody from NAG nor F (ab')₂ of antithyroglobulin antibody obtained from other 4 patients reacted with the idiotypic antiserum.<br>2) Binding of the idiotypic antiserum to NAG F (ab')₂ of anti-thyroglobulin antibody was inhibited by NAG IgG and NAG IgG F (ab')₂ with anti-thyroglobulin antibody but not by NAG F (ab')₂ without anti-thyroglobulin antibody and pooled human IgG.<br>The reaction between anti-thyroglobulin antibody and idiotypic antiserum was inhibited by thyroglobulin till 45 percent of control. No cross reaction was detected between the idiotypic antiserum and different IgG obtained from 9 patients with chronic thyroiditis.