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I read the book titled “that day” in Japanese written by Mis Obokata.
The book contains Obokata's memories during working as a researcher in Riken Wakayama laboratory in Kobe.
STAP story had many mysterious events, finally she was accused to be the most suspicious person who contaminated ES cell. Offical committee did not comfirm her guilty of ES contamination, but committee's conclusion harmed her seriously. As the result she suffered from depression. Therefore she decided to publish her book describing about her experience in Riken after 2 years of the miscondut event.
She wrote her book as memories style, but, actually her book asked Dr Wakayama to reveal his manipulation about STAP experiments. The most important points in her book is that she did not participate making STAP stem cells.
At first, she asked Dr Wakayama to reveal what kinds of mouse strain she was given prior to STAP cell preparing. She wrote in her book that she used the mice just she was given from Dr Wakayama without knowing its strain, therefore, she did not check its strain at all. In the past press conference at June 16 in 2014, Dr Wakayama said he himself or other staffs in the laboratory prepared the mouse. For preparing STAP cells, she just used the mouse given by Dr Wakayama or other staffs in the laboratory.
Initially, Dr Wakayama passed her the mouse directly, later, other staff did so. Dr Wakayama said he did not leave any mouse preparing records. Katsura official investigation committee confirmed Obokata did not know any mice strain at all. Further, she was confused why the officially analyzed results of STAP (stem) cell gene differed from those of mouse she used in the experiment. She was not able to mention the reason of these gaps. She wrote Dr Wakayama was the only parson who could tell the reason because he made STAP stem cell alone.
In the course of STAP experiments, Dr Wakayama succeeded STAP stem cells conversion from STAP cells. He succeeded STAP stem cell experiments in a row.
Therefore, Obokata's part of STAP cell experiments was exclusively restricted. She just made STAP cell through lethal processing with acid stress by using spleen lymphocytes taken from young mice. Dr Wakayama made STAP stem cell named FLS. Then he made Fgf induce stem cells named CTS or FI which were able to make fetus and placenta. She wrote that STAP stem cell experiments were done by Dr Wakayama himself without Obokata’s Cooperation.
She was not thought how to use manipulator at all in Wakayama laboratory, therefore she did not make STAP stem cells. After all, she was not allowed to participate STAP stem cell and related experiments.
She tried to increase STAP cell by adapting STAP cell to artificial medium but failed. For her, it was almost impossible to culture STAP cells in the artificial ES or TS medium.
Actually, she continued to struggle with STAP cells culturing without success.
According to the contract, Wakayama laboratory moved to the new laboratory in Yamanasi University in March 2013.
After Wakayama laboratory leaving, Dr Sasai helped Obokata to finish the rewrote version of STAP manuscript to Nature. Then, Mis Obokata sent the finished version of the manuscript to Nature in March 2013.
Nature requested her to send the STAP manuscript after correction of several pointed contents. She struggled to make revised version of STAP manuscript according to Dr Sasai and Dr Niwa’s advises.
Unfortunately, she was not able to contact Dr Wakayama who actually conducted STAP stem cell experiments. Finally, Nature accepted STAP manuscript in December 2013.
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とりあえず、書いてみました。世界のどなたかがアクセスしてくれることが懸念されるので・・・。
問題ある部分だらけと思いますが、コメントいただければ、秘密裡に直しますのでご指摘ください。
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2018/4/6(金) 午後 11:58
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