April 5, 2016
Additional Information
ATP solution
Added: **ATP weight is shown not a theoretical value but a measured value which produced the best results. ATP weight was determined based on a change of the pH value.
April 3, 2016
Correction
Method 11
Corrected: “Add 500ml of HBSS and suspend a cell pellet using a 1000μl-pipette***” to “Add 500μl of HBSS and suspend a cell pellet using a 1000μl-pipette***”
April 2, 2016
Correction
ATP solution 1
Corrected: “Dilute 110.57mg of ATP into 1ml of H2O（200nM in H2O）” to “Dilute 110.57mg of ATP into 1ml of H2O（200mM in H2O）”
*It was confirmed that ATP (Adenosin5’-triphosphate disodium salt hydrate) is soluble in water at this concentration.