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Welcome to Protocol Exchange from Nature Protocols. The Protocol Exchange is an open resource where the community of scientists pool their experimental know-how to help accelerate research. Discover the protocols, share a protocol, join a lab group, comment on protocols, organize your favorites and personalize your experience.

A protocol for in vivo detection of reactive oxygen species

Authors: Edward Owusu-Ansah, Amir Yavari, Utpal Banerjee

Lab groups: Banerjee Lab (University of California)
- Associated Publications:
  - Distinct mitochondrial retrograde signals control the G1-S cell cycle checkpoint
2’,7’-dichlorofluorescein (H2DCF) and Dihydroethidium (DHE), have been used extensively in tissue culture experiments to evaluate reactive oxygen species (ROS) production. However, i...
Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation

Authors: Laura Pacey KK, Shelley Stead, Jacqueline Gleave A, Kasia Tomczyk, Laurie Doering C

Lab groups: Department of Pathology and Molecular Medicine, McMaster University
Essential technical tips for STAP cell conversion culture from somatic cells

Authors: Haruko Obokata, Yoshiki Sasai, Hitoshi Niwa

Lab groups: STAP Group RIKEN CDB
- Associated Publications:
  - Stimulus-triggered fate conversion of somatic cells into pluripotency
  - Bidirectional developmental potential in reprogrammed cells with acquired pluripotency
THE AUTHORS ARE RETRACTING THIS PROTOCOL EXCHANGE PROTOCOL BECAUSE THE PAPERS UPON WHICH IT IS BASED HAVE BEEN RETRACTED. Stimulus-triggered acquisition of pl...

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Anisotropic Mobilities in Organic Semiconductors

Authors: Keli Han, Jindou Huang, Shuo Chai, Shuhao Wen, Weiqiao Deng

Lab groups: Han's Lab, Dalian Institute of Chemical Physics
- Associated Publications:
As one of the most important physical properties of the organic material, the anisotropic charge-carrier mobility directly determines device performance to a large extent. In this protocol, we main...
Production of neuron-preferential lentiviral vectors

Authors: Takashi Torashima, Chiho Koyama, Haruhiro Higashida, Hirokazu Hirai

Lab groups: H. Higashida Lab (Kanazawa Univ)
- Associated Publications:
  - CD38 is critical for social behaviour by regulating oxytocin secretion
Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells 1, 2 and are toxic to infected cells. Alternatively, the use of lentiviral vectors for t...
Enhancing graphene/CNT based electrochemical detection using magneto-nanobioprobes

Authors: Priyanka Sharma, V Bhalla, E Senthil Prasad, V Dravid, G Shekhawat, C. Raman Suri

Lab groups: Nanobiotech and Biosensor Lab, IMTECH, Chandigarh
- Associated Publications:
  - Enhancing electrochemical detection on graphene oxide-CNT nanostructured electrodes using magneto-nanobioprobes
This protocol describes an optimized signal amplification strategy to develop an ultra-sensitive magneto-electrochemical biosensing platform. The new protocol combines the advantages of carbon nano...

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Reconstitution of transmembrane protein Na+,K+-ATPase in giant unilamellar vesicles of lipid mixtures involving PSM, DOPC, DPPC and cholesterol at physiological buffer and temperature conditions

Authors: Tripta Bhatia, Flemming Cornelius, Ole G. Mouritsen, John H. Ipsen

Lab groups: Ipsen's Lab, MEMPHYS.

We prepare compositionally uniform samples of giant unilamellar vesicles (GUVs) of two model rafts mixtures: DOPC/DPPC/cholesterol and DOPC/PSM/chol lipid mixtures with and without transmembane pro...
Fabrication of a Corneal Epithelial Cell Sheet from Human Pluripotent Stem Cells by a Method Based on Spontaneous Ocular Cell Differentiation

Authors: Ryuhei Hayashi, Yuki Ishikawa, Ryosuke Katori, Yuki Taniwaki, Motokazu Tsujikawa, Andrew J. Quantock, Kohji Nishida

Lab groups: Stem Cells and Ophthalmology group, Osaka University

We have established a novel culture system to induce the formation of corneal epithelium through the spontaneous development of ocular cells from human induced pluripotent stem cells (hiPSCs). The ...
Effect of pegylated nano niosomal 2, 4- dichlorophenoxyacetic acid on intracellular taxol production

Authors: Leila jabbari, Sepideh Torabi, Afshin Peirovi, Mohsen Chiani, Farhad Masoomi-Aladizgeh, Dariush Norouzan

Lab groups: Nano-Biotechnology Dept.,Pasteur Institute of Iran

Nowadays, studies about yew tree as a main source of taxol (anticancer drugs) is being expanded rapidly. In the last few years, taxol is being isolated from the bark of Taxus brevifolia as the spe...