Video 2: Live imaging of low-pH-treated CD45+cells (another view)
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- Haruko Obokata,1, 2, 3
- Teruhiko Wakayama,3, 8
- Yoshiki Sasai,4
- Koji Kojima,1
- Martin P. Vacanti,1, 5
- Hitoshi Niwa,6
- Masayuki Yamato7
- & Charles A. Vacanti1
- Journal name:
- Nature
- Volume:
- 505,
- Pages:
- 641–647
- Date published:
- DOI:
- doi:10.1038/nature12968
Streaming video does not reproduce the original video quality. Download the video using the link below to view at original quality.
DIC images during day 0 – day 6, overlaid with oct3/4::GFP (green). The interval of imaging was half (15 min) of that of video 1 (the overall speed of the video is three-times slower than video 1). In this view field where the cell density was relatively low, behaviours of individual cells were more easily seen. In this case, forming clusters were slightly smaller in size.More videos from this article
Additional data
Affiliations
-
Laboratory for Tissue Engineering and Regenerative Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA
- Haruko Obokata,
- Koji Kojima,
- Martin P. Vacanti &
- Charles A. Vacanti
-
Laboratory for Cellular Reprogramming, RIKEN Center for Developmental biology, Kobe 650-0047, Japan
- Haruko Obokata
-
Laboratory for Genomic Reprogramming, RIKEN Center for Developmental biology, Kobe 650-0047, Japan
- Haruko Obokata &
- Teruhiko Wakayama
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Laboratory for Organogenesis and Neurogenesis, RIKEN Center for Developmental biology, Kobe 650-0047, Japan
- Yoshiki Sasai
-
Department of Pathology, Irwin Army Community Hospital, Fort Riley, Kansas 66442, USA
- Martin P. Vacanti
-
Laboratory for Pluripotent Stem Cell Studies, RIKEN Center for Developmental biology, Kobe 650-0047, Japan
- Hitoshi Niwa
-
Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo 162-8666, Japan
- Masayuki Yamato
-
Present address: Faculty of Life and Environmental Sciences, University of Yamanashi, Yamanashi 400-8510, Japan.
- Teruhiko Wakayama
Contributions
H.O. and Y.S. wrote the manuscript. H.O., T.W. and Y.S. performed experiments, and K.K. assisted with H.O.’s transplantation experiments. H.O., T.W., Y.S., H.N. and C.A.V. designed the project. M.P.V. and M.Y. helped with the design and evaluation of the project.
Competing financial interests
The authors declare no competing financial interests.
Author details
Haruko Obokata
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Teruhiko Wakayama
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Yoshiki Sasai
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Koji Kojima
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Martin P. Vacanti
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Hitoshi Niwa
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Masayuki Yamato
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Charles A. Vacanti
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Extended Data Figure 1: Conversion of haematopoietic cells into Oct4-GFP+ cells by a low-pH exposure.Hover over figure to zoom
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Extended Data Figure 2: Phenotypic change during STAP cell conversion.Hover over figure to zoom
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Extended Data Figure 3: Gene expression analyses during STAP conversion and endoderm differentiation assay.Hover over figure to zoom
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Extended Data Figure 4: Teratoma formation assay and characterization of Oct4-GFP-dim cells.Hover over figure to zoom
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Extended Data Figure 5: In vitro characterization of STAP cells.Hover over figure to zoom
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Extended Data Figure 6: Conversion of somatic tissue cells into STAP cells.Hover over figure to zoom
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Extended Data Figure 7: Generation chimaeras with STAP cells.Hover over figure to zoom
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Extended Data Figure 8: Molecular and cellular characterization of STAP stem cells.Hover over figure to zoom
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Extended Data Figure 9: Effects of various stressors on STAP conversion.Hover over figure to zoom
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Video 1: Live imaging of low-pH-treated CD45+cells
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Video 2: Live imaging of low-pH-treated CD45+cells (another view)
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Video 3: STAP cell-derived embryo (E10.5) from 4N blastocyst injection
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Video 4: Beating cardiac muscle generated from STAP-SCs in vitro Bright-field image.