2011年12月6日更新
2011年12月6日更新
論文業績
Suga, H., Kadoshima, T., Minaguchi, M., Ohgushi, M., Soen, M.,Nakano, T., Takata, N., Wataya, T., Muguruma, K., Miyoshi, H., Yonemura, S., Oiso, Y. & Sasai, Y. (2011) Self-formation of functional adeno-hypophysis in three-demensional culture. Nature 480, 57−62
Eiraku, M., Adachi, T. and Sasai, Y. (2012) Relaxation-Expansion Model for Self-Driven Retinal Morphogenesis. BioEssays 34, doi: 10.1002/bies.201100070. [Epub ahead of print].
Eiraku, M., Takata, N., Ishibashi, H., Kawada, M., Sakakura, E., Okuda, S., Sekiguchi, K., Adachi, T. and Sasai, Y. (2011) Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature 472, 51-56.
Kamiya, D., Banno, S., Sasai, N., Watanabe, K., Kawada, M., Yakura, R., Jakt , L.M., Nishikawa, S. and Sasai. Y. (2011) Intrinsic transition of ES cell differentiation into neural progenitors. Nature 470, 503-509
Ohgushi, M. and Sasai, Y. (2011) Lonely death dance of human pluripotent stem cells: ROCKing between metastable cell states. Trends in Cell Biology (in press)
Danjo, T., Eiraku, M., Muguruma, K., Watanabe, K., Kawada, M., Yanagawa, Y., Rubenstein, J.L.R. and Sasai, Y. (2011) Subregional specification of ES cell-derived ventral telencephalic tissues by timed and combinatory treatment with extrinsic signals. J. Neurosci. 31, 1919-1933
Muguruma, K., Nishiyama, A., Ono, Y., Miyawaki, H., Mizuhara, E., Hori, S., Kakizuka, A., Obata, K., Yanagawa, Y., Hirano, T. and Sasai, Y. (2011) Ontogeny-recapitulating generation and tissue integration of ES cell-derived cerebellar Purkinje cells. Nature Neurosci 13, 1171 - 1180
Aramaki, T., Sasai, N., Yakura, R. and Sasai, Y. (2010) Jiraiya Attenuates BMP Signaling by Interfering with Type-II BMP Receptors in Neuroectodermal Patterning. Developmental Cell 19, 547-561
Ohgushi, M., Matsumura, M., Eiraku, M., Murakami, K., Aramaki, T., Nishiyama, A., Muguruma, K., Nakano, T., Suga, H., Ueno, M., Ishizaki, T., Suemori, H., Narumiya, S., Niwa, H. and Sasai, Y. (2010) Molecular Pathway and Cell State Responsible for Dissociation-Induced Apoptosis in Human Embryonic Stem Cells. Cell Stem Cell 7, 225-239
Takai, A., Inomata, H., Arakawa, A., Matsuo-Takasaki, M. and Sasai, Y. (2010) Anterior neural development requires Del1, a matrix-associated protein that attenuates canonical Wnt signaling via the Ror2 pathway. Development 137, 3293-3302
Eiraku, M, Watanabe, K., Matsuo-Takasaki, M., Kawada, M., Yonemura, S., Matsumura, M., Wataya, T., Nishiyama, A., Muguruma, K. and Sasai, Y. (2008) Self-Organized Formation of Polarized Cortical Tissues from ES cells and its Active Manipulation by Extrinsic Signals. Cell Stem Cell 3, 519-532
Inomata, H., Haraguchi, T. and Sasai, Y. (2008) Robust Stability of the Embryonic Dorsal Axial Pattern Requires ONT1, a Secreted Scaffold for Chordin Degradation. Cell 134, 854-865.
Wataya, T., Ando, S., Muguruma, K., Ikeda, H., Watanabe, K., Eiraku, M., Kawada, M., Takahashi, J., Hashimoto, N. and Sasai, Y. (2008) Minimization of Exogenous Signals in ES Cell Culture Induces Rostral Hypothalamic Differentiation. Proc Natl Acd Sci USA 105, 11796-11801.
Sasai, N., Yakura, R,, Kamiya, D., Nakazawa, Y. and Sasai, Y. (2008) Ectodermal Factor Restricts Mesoderm Differentiation by Inhibiting p53. Cell 133, 878-890
Osakada, F., Ikeda, H., Mandai, M., Wataya, T., Watanabe, K., Yoshimura, N., Akaike, A., Sasai, Y, Takahashi M. (2008) Toward ｇeneration of rod- and cone-photoreceptors from human and monkey embryonic stem cells under defined culture conditions. Nature Biotechnology 26, 215-24
Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S.-i., Muguruma, K. and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nature Biotechnology 25, 681 - 686
Onai, T., Matsuo-Takasaki, M., Inomata, H., Aramaki, T., Matsumura, M., Yakura, R., Sasai, N., and Sasai, Y. (2007) XTsh3 is an essential enhancing factor of canonical Wnt signaling in Xenopus axial determination EMBO J 26, 2350-2360.
Arakawa, A., Matsuo-Takasaki, M., Takai, A., Inomata, H., Matsumura, M., Ikeya, M., Takahashi, K., Miyachi, Y., Sasai, N. and Sasai,Y. (2007) The secreted EGF-Discoidin factor xDel1 is essential for dorsal development of the Xenopus embryo. Developmental Biology 306, 160-169
Ikeya, M., Kawada, M., Kiyonari, H., Sasai, N., Nakao, K., Furuta, Y. and Sasai, Y. (2006) Essential Pro-BMP Roles of Crossveinless 2 in Mouse Organogenesis. Development 133, 4463-4473.
Ueno, M., Matsumura, M., Watanabe, K., Nakamura, T., Osakada, F., Takahashi, M., Kawasaki, H., Kinoshita, S., and Sasai, Y. (2006) Neural Conversion of Embryonic Stem Cells by an Inductive Activity on Human Amniotic Membrane Matrix Proc. Natl. Acad. Sci. USA 103, 9554-9559
Brederlau, A., Correia, A.S., Anisimov, S.V., Elmi, M., Paul, G., Roybon, L., Morizane, A., Bergquist, F., Riebe, I., Nannmark, U., Carta, M., Hanse, E., Takahashi, J., Sasai, Y., Funa, K., Brundin, P., Eriksson, P.S., Li, J.Y. (2006) Transplantation of human embryonic stem cell-derived cells to a rat model of Parkinson's disease: effect of in vitro differentiation on graft survival and teratoma formation. Stem Cells 24, 1433-1430
Morizane A, Takahashi J, Shinoyama M, Ideguchi M, Takagi Y, Fukuda H, Koyanagi M, Sasai Y, Hashimoto N. (2006) Generation of graftable dopaminergic neuron progenitors from mouse ES cells by a combination of coculture and neurosphere methods. J Neurosci Res. 83, 1015-27.
Yamazoe, H., Kobori, M., Murakami, Y., Yano, K., Satoh, M., Mizuseki, K., Sasai, Y., & Iwata, H. (2006) One-Step Induction of Neurons From Mouse Embryonic Stem Cells in Serum-Free Media Containing Vitamin B12 and Heparin. Cell Transplantation, 15, 135-145
Su, H.-L., Muguruma, K., Kengaku, M., Matsuo-Takasaki, M., Watanabe, K., and Sasai, Y. (2006) Generation of Cerebellar Neuron Precursors from Embryonic Stem Cells. Developmental Biology 290, 287-296.
Ikeda, H., Watanabe, K., Mizuseki, K., Haraguchi, T., Miyoshi, H., Kamiya, D., Honda, Y., Sasai, N., Yoshimura, N., Takahashi, M. and Sasai, Y. (2005) Generation of Rx+/Pax6+ neural retinal precursors from embryonic stem cells. Proc. Natl. Acad. Sci. USA 102, 11331-11336
Sakuragi, M., Sasai, N., Ikeya, M., Kawada, M., Katahira, T., Nakamura, H. and Sasai, Y. (2006) Functional Analysis of Chick ONT1 Reveals Distinguishable Activities among Olfactomedin-Related Signaling Factors. Mech. Dev. 123, 114-123.
Morizane, A., Takahashi, J., Shinoyama, M., Ideguchi, M., Takagi, Y., Fukuda, H., Koyanagi, M., Sasai, Y. and Hashimoto, N. (2006) Generation of graftable dopaminergic neuron progenitors from mouse ES cells by a combination of co-culture and neurosphere methods. Journal of Neuroscience Research 83, 1015-1027.
Fukuda H, Takahashi J, Watanabe K, Hayashi H, Morizane A, Koyanagi M, Sasai Y, Hashimoto N. (2006) FACS-based purification of ES cell-derived neural precursors averts tumor formation after transplantation. Stem cells 24,763-771
Matsuo-Takasaki, M., Matsumura, M. and Sasai, Y. (2005) An essential role of Xenopus Foxi1a for ventral specification of the cephalic ectoderm during gastrulation Development 132, 3885-3894
Ikeya, M., Kawada, M., Nakazawa, Y., Sakuragi, M., Sasai, N., Ueno, M., Kiyonari, H., Nakao, K. and Sasai, Y. (2005) Gene Disruption/Knock-in Analysis of mONT3: Vector Construction by Combining in vivo and in vitro Recombinations. Int. J. Dev. Biol. 49, 807-823
Sato, T., Sasai., N. and Sasai, Y. (2005) Neural Crest Determination by Co-activation of Pax3 and Zic1 Genes in Xenopus Ectoderm. Development 132, 2355-2363
Yamazoe H, Murakami Y, Mizuseki K, Sasai Y, and Iwata H. (2005) Collection of neural inducing factors from PA6 cells using heparin solution and their immobilization on plastic culture dishes for the induction of neurons from embryonic stem cells. Biomaterials 26, 5746-54.
Watanabe, K., Kamiya, D., Nishiyama, A., Katayama, T., Nozaki, S., Kawasaki, H., Mizuseki, K., Watanabe, Y., and Sasai, Y. (2005) Directed differentiation of telencephalic precursors from embryonic stem cells. Nature Neurosci. 8, 288-296
Takagi, Y., Takahashi, J., Saiki, H., Morizane, A., Hayashi, T., Kishi, Y., Fukuda, H., Okamoto, Y., Koyanagi, M., Ideguchi, M., Hayashi, H., Imazato, T., Kawasaki, H., Suemori, H., Omachi, S., Iida, H., Itoh, N., Nakatsuji, N., Sasai, Y. and Hashimoto, N. (2005) Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model. J. Clin. Invest. 115, 102-109
Irioka, T., Watanabe, K., Mizusawa, H., Mizuseki, and Sasai, Y. (2005) Distinct effects of caudalizing factors on regional specification of embryonic stem cell-derived neural precursors. Dev. Brain Res. 154, 63-70
Onai, T., Sasai, N., Matsui, M. and Sasai, Y. (2004) Xenopus XsalF: Anterior Neuroectodermal Specification by Attenuating Cellular Responsiveness to Wnt Signaling. Dev. Cell 7, 95-106
Sasai, N., Nakazawa, Y., Haraguchi, T. and Sasai, Y. (2004) The Neurotrophin Receptor-related Protein NRH1 is Essential for Convergent Extension Movements. Nature Cell Biol. 6, 741-748.
Haruta, M., Sasai, Y., Kawasaki, H., Amemiya, K., Ooto, S., Kitada, M., Suemori, H., Nakatsuji, N., Ide, C., Honda, Y. and Takahashi, M. (2004) In vitro and in vivo characterization of pigment epithelial cells differentiated from primate embryonic stem cells. Invest. Ophthalmol. Vis. Sci. 45, 1020-1025
Mizuseki, K., Sakamoto, T., Watanabe, K., Muguruma, K., Ikeya, M., Nishiyama, A., Arakawa, A., Suemori, H., Nakatsuji, N., Kawasaki, H., Murakami, F. and Sasai, Y. (2003) Generation of Neural Crest-Derived PNS Neurons and Floor Plate Cells from Mouse and Primate ES Cells. Proc. Natl. Acad. Sci. USA 100, 5828-5833
Tada, M., Morizane, A., Kimura, H., Kawasaki, H., Ainscough, J.F.X., Sasai, Y., Nakatsuji, N. and Tada, T. (2003) Pluripotency of reprogrammed somatic genomes in ES hybrid cells. Dev. Dyn. 227, 504-510
Ooto, S., Haruta, M., Honda, Y., Kawasaki, H., Sasai, Y. and Takahashi, M. (2003) Induction of the differentiation of lentoids from primate embryonic stem cells. Invest. Ophthalmol. Vis. Sci, 44, 2689-2693.
Morizane A, Takahashi J, Takagi Y, Sasai Y, Hashimoto N. (2002)
Optimal conditions for in vivo induction of dopaminergic neurons from embryonic stem cells through stromal cell-derived inducing activity. J Neurosci Res. 69、934-939
Tsuda,H., Sasai, N., Matsuo-Takasaki, M, Sakuragi, M., Murakami, Y. and Sasai, Y. (2002) Dorsalization of the Neural Tube by Xenopus Tiarin, a Novel Patterning factor Secreted by the Flanking Non-Neural Head Ectoderm. Neuron 33,515-528.
Kawasaki, H., Suemori, H., Mizuseki, K., Watanabe, K., Urano, F., Ichinose, H., Haruta, M., Takahashi, M., Yoshikawa, K., Nishikawa, S.i., Nakatsuji, N. and Sasai, Y. (2002) Generation of TH+ Dopaminergic Neurons and Pax6+ Pigment Epithelia from Primate ES cells by SDIA. Proc. Natl. Acad. Sci. USA 99, 1580-1585
Kawasaki, H., Mizuseki, K. & Sasai, Y. Selective neural induction from ES cells by stromal cell-derived inducing activity and its possible application to parkinsonian therapy. In Methods in Molecular Biology 185, 217-228 (edited by Kursad Turksen, Humana Press, Totowa, NJ), 2001
Sasai, N., Mizuseki, K. and Sasai, Y. (2001) Requirement of FoxD3-class Signaling for Neural Crest Determination in Xenopus. Development 128, 2525-2536
Sasai, Y.(2000) Regulation of neural determination by evolutionally conserved signals: anti-BMP factors and what next? Curr. Opin. in Neurobiol. 11, 22-26
Kawasaki, H., Mizuseki, K. , Nishikawa, S., Kaneko, S., Kuwana, Y., Nakanishi, S., Nishikawa, S.-I. and Sasai, Y. (2000) Induction of midbrain dopaminergic neurons from ES cells by Stromal Cell-Derived Inducing Activity. Neuron 28, 31-40.
Matsui, M., Mizuseki, K., Nakatani, J., Nakanishi, S. and Sasai, Y. (2000)
Xenopus Kielin: A novel patterning factor containing multiple Chd-type repeats secreted from the embryonic midline. Proc. Natl. Acad. Sci. USA 97, 5291-5296
Kishi, M., Mizuseki, K., Sasai, N., Yamazaki, H., Shiota, K., Nakanishi, S. and Sasai, Y. (2000) Requirement of Sox2-mediated Signaling for Differentiation of Early Xenopus Neuroectoderm. Development 127, 791-800
Nakatani, J., Mizuseki, K., Tsuda, H., Nakanishi, S. and Sasai, Y. (2000)
Xenopus Xenf: An early endodermal nuclear factor that is regulated in a pathway distinct from Sox17 and Mix-related gene pathways Mech. Dev. 91, 81-89
Sasai, Y. (1998) Identifying the missing links: genes that connect neural induction and primary neurogenesis in vertebrate embryos. Neuron 21, 455-458
Mizuseki, K., Kishi, M., Shiota, K., Nakanishi, S. and Sasai, Y. (1998) Sox-D is an essential mediator for induction of anterior neural tissues in Xenopus embryos. Neuron 21, 77-85.
Mizuseki, K., Kishi, M., Matsui, M., Nakanishi, S. and Sasai, Y. (1998) Xenopus Zic-related-1 and Sox-2, two factors inducer by Chordin, have distinct activities in the initiation of neural induction. Development 125 , 579-587.
Yamanaka, Y., Mizuno, T., Sasai, Y., Kashi, M., Takeda, H., Hibi, M. and Hirano, T. (1998) A novel zebrafish homeobox gene, dharma, induces the organizer in a non-cell-autonomous manner. Genes and Dev. 12, 2345-2353
Sasai.Y. and De Robertis, E.M. (1997) Ectodermal patterning in vertebrate embryos. Developmental Biology 182, 5-20.
Sasai, Y., Lu, B., Stefano, S. and De Robertis, E.M. (1996) Endoderm induction by the organizer-secreted factors Chordin and Noggin in Xenopus animal caps. EMBO J. 16, 4547-4555.
Piccolo, S., Sasai, Y., Lu, B., and De Robertis, E.M. (1996) Dorsoventral patterning in Xenopus: Inhibition of ventral signals by direct binding of Chordin to BMP-4. Cell 86, 589-598.
Holley, S.A., Neul, J.L., Attisano, L., Wrana, J.L., Sasai, Y., Y., O'Connor, M.B., De Robertis, E.M. and Frguson, E.L. (1996) The Xenopus dorsalizing factor noggin ventralizes Drosophila embryos by preventing Dpp from activating its receptor. Cell 86, 607-792.
Bouwmeester, T., Kim, S.-H., Sasai, Y., Lu , B. and De Robertis, E.M. (1996) Cerberus is a head-inducing secreted factor expressed in the anterior endoderm of Spemann's organizer. Nature 382, 595-601.
De Robertis, E.M. and Sasai, Y. (1996) A common plan for dorsoventral patterning in Bilateria. Nature 380, 37-40.
Sasai, Y., Lu, B., Steinbeisser, H. and De Robertis, E.M. (1995) Regulation of neural induction by the chd and BMP-4 antagonistic patterning signals in Xenopus. Nature 376, 333-336
Holley, S.A., Jackson, P.D., Sasai, Y.(the first three authors are equal contributors to this work), Lu, B., De Robertis, E.M., Hoffmann, F.M. and Ferguson, E.L. (1995) A conserved system for dorsal-ventral patterning in insects and vertebrates involving short gatrulation and chordin. Nature 376, 249-253
Sasai Y., Lu, B., Steinbeisser H., Geissert D., Gont L. and De Robertis E. M. (1994) Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes. Cell 79, 779-790
Sasai, Y., Kageyama, R., Tagawa, Y.,Shigemoto, R. and Nakanishi, S.(1992)
Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split. Genes and Development 12B,2620-34.
Ishibashi, M., Moriyoshi, K., Sasai, Y., Shiota, K., Nakanishi, S. and Kageyama, R. (1994) Persistent expression of helix-loop-helix factor HES-1 prevents mammalian neural differentiation in the central nervous system. EMBO J. 13, 1799-1805.
Takebayashi, K., Sasai, Y., Sakai, Y., Watanabe, T., Nakanishi, S. and Kageyama, R. (1994) Structure, chromosomal locus, and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-1. Negative autoregulation through the multiple N box elements. J. Biol. Chem. 269, 5150-6
Akazawa, C., Sasai, Y., Nakanishi, S. and Kageyama R.(1992) Molecular characterization of a rat negative regulator with a basic helix-loop-helix structure predominantly expressed in the developing nervous system. J. Biol. Chem. 267, 21879-85.
Kageyama, R., Sasai, Y. and Nakanishi, S.(1991)Molecular characterization of transcription factors that bind to the cAMP responsive region of the substance P precursor gene. cDNA cloning of a novel C/EBP-related factor J. Biol. Chem. 266, 15525-31.
Sasai, Y. and Nakanishi, S. (1989) Molecular characterization of rat substance K receptor and its mRNAs. Biochem. Biophy. Res. Comm. 165, 695-702.
Yokota, Y., Sasai, Y., Tanaka, K., Fujiwara, T., Tsuchida, K., Shigemoto, R., Kakizuka, A., Ohkubo, H. and Nakanishi S. (1989) Molecular characterization of a functional cDNA for rat substance P receptor. J. Biol. Chem. 264, 17649-52.
Nakanishi, S., Ohkubo, H., Kakizuka, A., Yokota, Y., Shigemoto, R., Sasai Y. and Takumi T.(1990) Molecular characterization of mammalian tachykinin receptors and a possible epithelial potassium channel. Recent Progress in Hormone Research 46:59-83
Kashii, S., Ito, K., Monden, S., Sasai, Y., Tsuchida, K., Fujita, M., Kawamoto, H., Norioka, M., Okuma, M. (1991) Adenosine deaminase deficiency due to heterozygous abnormality consisting of a deletion of exon 7 and the absence of enzyme mRNA. J.Cell. Biochem 47, 49-53.
Kawamoto, H., Ito, K., Kashii, S., Monden, S., Fujita, M., Norioka, M,. Sasai, Y., Okuma, M. (1993) A point mutation in the 5' splice region of intron 7 causes a deletion of exon 7 in adenosine deaminase mRNA. J.Cell. Biochem 51, 322-5.
Takai. K, Sasai, Y., Morimoto, H., Yamazaki, H., Yoshii, H. and Inoue S.(1984) Enzymatic dehydrogenation of tryptophan residues of human globins by tryptophan side chain oxidase II. J. Biol. Chem. 259, 4452-7.