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Nucleic Acids Research, 1979, Vol. 6, No. 5 1831-1842
© 1979

Articles

DNA sequences of the integration sites and "inverted repeated structure of transposon Tn3

Tatsuo Takeya, Hisayuki Nomiyama, Miyoshi Jun, Shimada Kazunori and Yasuyuki Takagi

Department of Biochemistry, Kyushu University School of Medicine Fukuoka, Japan

Received February 21, 1979. The nucleotide sequence of the "inverted repeat" structure of the transposon Tn3 was determined by the DNA sequencing procedure developed by Maxam and Gilbert(1). The sequence, 38 base pairs long, is as follows:

5'-GGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAG.(Tn3) 3'-CCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTC.

The integration of Tn3. is associated with a directly repeated sequence of 5 nucleotides appearing at each end of Tn3. The two directly repeated sequences so far determined are not the same. Furthermore; there is no homologous structure around the integration point of Tn3.


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