Table of Contents

 

Invited Oral Presentations

S01Probing nanostructures on cell surfaces - the route to understanding cell membrane organization and function

Prof. Michael Edidin (Johns Hopkins Univ.)

マイケル・エディディン(ジョンズ・ホプキンス大学)········································ 1

 

S02Scanning probe microscopic analyses of chromosome function and structure

Prof. Tatsuo Ushiki (Niigata Univ.)

牛木辰男(新潟大学医学部)···································································· 4

 

S03Exploring the restless nano-architecture of the cell membrane in living cells: Single molecules' approach

Prof. Akihiro Kusumi (Nagoya Univ.)

楠見明弘(名古屋大学院・生命理学)·························································· 7

 

S04Single-Molecule Fluorometry for Nanobiotechnology

Dr. Mitsuru Ishikawa (JRCAT)

石川満(アトムテクノロジー研究体)························································· 10

 

S05Nanobiotechnology and bioelectronics

Prof. Eiichi Tamiya (JAIST)

民谷栄一(北陸先端大)······································································· 12

 

 

Poster Presentations

 

Bioimaging by AFM

P01Development and Application of Carbon Nanotube Probe to Atomic Force Microscopy

Kunio Takeyasu, Yu-suke Masamura and Shige H. Yoshimura

Graduate School of Biostudies, Kyoto Univ.

○竹安邦夫、正村祐介、吉村成弘(京大院生命科学工学)···································· 17

 

P02Nanostructural Biology of Gene Function

Yougo Maruyama, Shige H. Yoshimura and Kunio Takeyasu

Graduate School of Biostudies, Kyoto Univ.

○円山由郷、吉村成弘、竹安邦夫(京大院生命科学工学)···································· 18

 


P03Analysis of DNA-HMG2 Complex Using AFM

Masato Saito, Yuji Murakami, Kenji Yokoyama, Shin-ichi Tate, and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○斉藤真人、村上裕二、横山憲二、楯真一、民谷栄一(北陸先端大)························· 19

 

P04Single Molecule Analyses by TEM and AFM for Functional DNA Transaction Complexes

Tsurimoto, T.1, Shiomi, Y.1, Usukura, J.2, Masamura, K.3, Takeyasu, K.3, and Yoshikawa, H.1

1Nara Advanced Institute of Science and Technology, 2Department of Anatomy, Nagoya University, School of Medicine, 3Graduate School of Biostudies, Kyoto University

○釣本敏樹、塩見泰史、臼倉治郎、正村祐介、竹安邦夫、吉川寛(奈良先端大、名古屋大学医学部、京大院生命科学工学)································································ 20

 

P05Analysis of Higher-order Structure of Escherichia Coli Genome by Using Atomic Force Microscopy

Yu-suke Masamura1, Azam, Talukder Ali2, Akira Ishihama2,and Kunio Takeyasu1

1Graduate School of Biostudies, Kyoto University, 2Department of Molecular Genetics, National Institute of Genetics

○正村祐介、Azam, Talukder Ali、石浜明、竹安邦夫(京大院生命科学工学、国立遺伝学研究所)···························································································· 21

 

P06Nanostructural Biology of the Chromatin

Shige H. Yoshimura and Kunio Takeyasu

Graduate School of Biostudies, Kyoto Univ.

○吉村成弘、竹安邦夫(京大院生命科学工学)················································ 22

 

P07The Spiral Structure of Human Chromosomes Observed by Atomic Force Microscopy (AFM)

Kousuke Iwai, Osamu Hoshi, Jiro Hitomi, and Tatsuo Ushiki

Department of Anatomy and Histology, Faculty of Medicine, Niigata University

○岩井孔良、星治、人見次郎、牛木辰男(新潟大医)········································· 23

 

P08Structural Analysis of Chromosome Bands by Atomic Force Microscopy

Osamu Hoshi, Kousuke Iwai, Jiro Hitomi, and Tatsuo Ushiki

Department of Anatomy and Histology, Faculty of Medicine, Niigata University

○星治、岩井孔良、人見次郎、牛木辰男(新潟大医)········································· 24

 

P09AFM Manipulation and Imaging of Human Chromosomes

Ken-ichi Ogawa1, Masato Saito1, Yuji Murakami1, Tatsuo Ushiki2, and Eiichi Tamiya1

1Japan Advanced Institute of Science and Technology, 2Niigata University

○小川健一、斉藤真人、村上裕二、牛木辰男、民谷栄一(北陸先端大、新潟大医)·········· 25

 

 

P10High-resolution Imaging of Chromosome Structure Using AFM

-Effect of Protein Extraction on Chromosome Surface-

S. Sugiyama1, X. Liu2, T. Yoshino1, S. Hagiwara1, and T. Ohtani1

1National Food Research Institute, 2Japan Atomic Energy Research Institute

○杉山滋、劉新旗、吉野智之、萩原昌司、大谷敏郎(食品総合研究所、日本原子力研究所)· 26

 

P11DC Electric-field Induced DNA Stretching for AFM and Surface Chemical Study

J. M. Kim1, T. Ohtani1 and H. Muramatsu2

1National Food Research Institute, 2Seiko Instruments Inc.

キム・ジョンミン、大谷敏郎、村松宏(食品総合研究所、セイコーインスツルメンツ)··· 27

 

AFM Physics

P12Shadow Images of Plasmid DNA by Field Electron Emission from a Point Source

M. Hirade, K. Nakatani, S. Tate, T. Arai, and M. Tomitori

Japan Advanced Institute of Science and Technology

○平出雅人、仲谷幸司、新井豊子、富取正彦(北陸先端大)·································· 28

 

P13STM Study of a TiO2 (110) 1x2 Surface as an Oxide Substrate for Biological Materials

N. Edo, T. Arai and M. Tomitori

 Japan Advanced Institute of Science and Technology

○江戸暢子、新井豊子、富取正彦(北陸先端大)·············································· 29

 

P14Microfabrication of Silicon Tip for Atomic Force Microscope Application

Takayuki Wakayama, Katsuhisa Shibuya, Nobuya Iwata, and Shoji Yamada

Center for New Materials, Japan Advanced Institute of Science and Technology

○若山貴行、渋谷克久、岩田展也、山田省二(北陸先端大)·································· 30

 

P15Microfabrication of Gallium Arsenide Tip for Atomic Force Microscope Application

Nobuya Iwata, Takayuki Wakayama, Katsuhisa Shibuya, and Shoji Yamada

Center for New Materials, Japan Advanced Institute of Science and Technology

○岩田展也、若山貴行、渋谷克久、山田省二(北陸先端大)·································· 31

 

Advanced Probe Microscope

P16Imaging Green Fluorescent Protein-tagged Receptor Molecules in Cellular Membranes with Near-field Scanning Optical Microscope (NSOM)

Shinichiro Iwabuchi1, Jeeseong Hwang2 and Michael Edidin1

1Department of Biology, The Johns Hopkins University, 2Optical Technology Division, National Institute of Standards and Technology

○岩渕紳一郎、Jeeseong Hwang、マイケル・エディディン(ジョンズ・ホプキンス大学、NIST································································································· 32

P17Characterization of Micro Optical Cantilever and Its Application to Biological Samples

Hiroshi Muramatsu1, Takashi Niwa1, Susumu Ichihara1, Yasuyuki Mitsuoka1, Jong-Min Kim2, Patrick Degenaar3, Hiromi Fujita3, Mitsuyoshi Ueda4, Eiichi Tamiya3

1Seiko Instruments, 2National Food Research Institute, 3Japan Advanced Institute of Science and Technology, 4Kyoto Univ.

○村松宏、新輪隆、市原進、光岡靖幸、キム・ジョンミン、パトリック・デガナー、藤田裕美、植田充美、民谷栄一(セイコーインスツルメンツ、食品総合研究所、北陸先端大、京大院工)·························································································· 33

 

P18SNOAM Imaging of GFP Displayed on Yeast Cell Surfaces

Hiromi Fujita, Mitsuyoshi Ueda*, Yasutaka Morita, and Eiichi Tamiya

Japan Advanced Institute of Science and Technology, Kyoto Univ. *

○藤田裕美、植田充美、森田資隆、民谷栄一(北陸先端大、京大院工)······················ 34

 

P19Near Field Fluorescent Imaging of Neurite Growths from Patterned Neuron

Patrick Degenaar, Laurent Griscom*, Yuji Murakami, Bruno Le Pfioufle*, Hiroaki Fujita*, and Eiichi Tamiya

Japan Advanced Institute of Science and Technology, Tokyo University*

○パトリック・デガナー、Laurent Griscom、村上裕二、Bruno Le Pfioufle、藤田裕美、民谷栄一(北陸先端大、東大生研)·································································· 35

 

NMR, Fluorescence Microscope and related works

P20NMR and CD Analysis of the Short Basic Tail Following HMG-2B Domain in its Contribution to DNA Binding

Madoka Kanbayashi1, Jun-ichi Kurita1, Michiteru Yoshida2, and Shin-ichi Tate1

1Center for New Materials, Japan Advanced Institute of Science and Technology, 2Science University of Tokyo

○上林円、栗田順一、吉田充輝、楯真一(北陸先端大新素材センター、東京理科大基礎工)· 36

 

P21High Resolution NMR Structure Analysis of Two HMG-box Domains in Nucleosome Protein HMG-2

Kouji Nakatani1, Jun-ichi Kurita1, Masatsune Kainosho2, Michiteru Yoshida3, and Shin-ichi Tate1

1Center for New Materials, Japan Advanced Institute of Science and Technology,

2Tokyo Metropolitan University, 3Science University of Tokyo

○仲谷幸司、栗田順一、甲斐荘正恒、吉田充輝、楯真一(北陸先端大新素材センター、東京都立大理、東京理科大基礎工)································································ 37

 

P223D Visualization of Gene Locations in a Barley Nucleus

Kiichi Fukui

Department of Biotechnology, Graduate School of Engineering, Osaka University

○福井希一(阪大院工)······································································· 38

 


P23Nuclear Volumes Revealed by 3D Analyses in Plants

Satoru Fujimoto, Mikako Ito and Kiichi Fukui

Department of Biotechnology, Graduate School of Engineering, Osaka University

○藤本聡、伊藤美佳子、福井希一(阪大院工)················································ 39

 

P24Determination of Genome and of Chromosomes Dimensions of Lotus Japonicus.

Mikako Ito1, Junko Miyamoto2, Yumiko Mori1, Satoru Fujimoto1, Toshiki Uchiumi2, Mikiko Abe2, Akihiro Suzuki2, Satoshi Tabata3, and Kiichi Fukui1

1Osaka University, 2Kagoshima University, 3Kazusa DNA Research Institute

○伊藤美佳子、宮本旬子、森裕美子、藤本聡、内海俊樹、阿部美紀子、鈴木章弘、田畑哲之、福井希一(阪大院工、鹿児島大、かずさDNA研究所)········································ 40

 

P25Isolation of Human Metaphase Chromosomes by the Flow-sorting Method

Takefumi Sone, Shouhei Kobayashi, Takeshi Ishihara, Yuki Mitsubayashi, and Kiichi Fukui

Department of Biotechnology, Graduate School of Engineering, Osaka University

○曽根岳史、小林昇平、石原武、三林有紀、福井希一(阪大院工)··························· 41

 

P26The Development of a Digital Image Database of Chromosomes

Junko Miyamoto1, Tamaki Hirose2, Yukio Akiyama3, Toshiyuki Wako4, and Kiichi Fukui5

1Kagoshima Univ. 2Japan Atomic Energy Research Institute, 3Japan Science and Technology Corporation, Hokuriku National Agricultural Experiment Station, 4National Institute of Agrobiological Resources, 5Graduate School of Engineering, Osaka University

○宮本旬子、廣瀬玉紀、秋山征夫、若生俊行、福井希一(鹿児島大理、日本原子力研究所、農林水産省北陸農業試験場、農林水産省農業生物資源研究所、阪大院工)···················· 42

 

P27Proteome Analysis of Human Chromosomes

Shouhei Kobayashi1, Takefumi Sone1, Takeshi Ishihara1, Yuki Mitsubayashi1, Toshifumi Takao2, Shinsuke Fujiwara1, Masahiro Takagi1, and Kiichi Fukui1

1Department of Biotechnology, Graduate School of Engineering, Osaka University,

2Institute for Protein Research, Osaka University

○小林昇平、曽根岳史、石原武、三林有紀、高尾敏文、藤原伸介、高木昌宏、福井希一(阪大院工、阪大蛋白研)·········································································· 43

 

Microarray, biosensor and related works

P28High Throughput Single Cell PCR on a Microchamber Array

Hidenori Nagai, Yuji Murakami and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○永井秀典、村上裕二、民谷栄一(北陸先端大)·············································· 44

 

 

 

P29In vitro Protein Synthesis on Microchamber Arrays

Takeshi Kinpara1, Hideo Nakano2, Yuji Murakami1, and Eiichi Tamiya1

1Japan Advanced Institute of Science and Technology, 2Nagoya University

○金原健、中野秀雄、村上裕二、民谷栄一(北陸先端大)···································· 45

 

P30Microfabricated Magnetic Particle as Bio-electrode on Array Chip

Yuji Murakami, Akira Yamamura, Yong-Song Choi, and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○村上裕二、山村晃、チェ・ヨンソン、民谷栄一(北陸先端大)····························· 46

 

P31High-integrated Biochip by Hydrophobic Interaction

Yong-Song Choi, Do-Kyun Kim, Yuji Murakami, and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○チェ・ヨンソン、キム・ドキュン、村上裕二、民谷栄一(北陸先端大)···················· 47

 

P32Fabrication of 2D Superlattices of Gold Nanoparticles

Toshiharu Teranishi and Mikio Miyake

Japan Advanced Institute of Science and Technology

○寺西利治、三宅幹夫(北陸先端大)························································· 48

 

P33Bioimaging of Neural Cell Network on Microfabricated Chamber Array

Yoshinori Akagi, Yuji Murakami and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○赤木良教、村上裕二、民谷栄一(北陸先端大)·············································· 49

 

P34Miniaturization and Integration of SPR Immunosensors

Masayasu Suzuki1, Fumihiko Ozawa1, Wakako Sugimoto2, Takehiro Murashige2, and Shuji Aso2

1Toyama University, 2Kyushu Institute of Technology

○鈴木正康、小沢文彦、杉本和歌子、村重剛弘、麻生秀司(富山大工、九州工業大情報工)· 50

 

P35Enzyme Electrochemistry Mediated by SAM-based Redox Active Polymer

Shu Taira, Eiichi Tamiya and Kenji Yokoyama

Japan Advanced Institute of Science and Technology

○平修、民谷栄一、横山憲二(北陸先端大)·················································· 51

 


Microfluidics for biological application

P36On-chip Bypass Structure for Sample Segment Dilution

Takashi Kanekiyo, Yuji Murakami and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○兼清崇、村上裕二、民谷栄一(北陸先端大)················································ 52

 

P37Electrochemical Gene Detection with PCR Chip

Masaaki Kobayashi1, Miyuki Oomura1, Takashi Kusakawa2, Yasutaka Morita1, Yuji Murakami1, Kenji Yokoyama1, and Eiichi Tamiya1

1Japan Advanced Institute of Science and Technology, 2Hokuto Science Industry

○小林正昭、大村美由紀、草川貴史、森田資隆、村上裕二、横山憲二、民谷栄一(北陸先端大、北斗科学産業)············································································ 53

 

P38Allergy Sensor with Mast Cell Cultivated on a Chip

Yasutaka Matsubara, Yuji Murakami and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○松原泰孝、村上裕二、民谷栄一(北陸先端大)·············································· 54

 

P39Integrated Microfluidic Devices for Chromosome Engineering

Takahito Inoue and Hiroshi Yokoyama

Electrotechnical Laboratory

○井上貴仁、横山浩(経済産業省電子技術総合研究所)······································· 55

 

P40On Chip Screening of Dioxin Binding Peptide

Yasutaka Morita and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○森田資隆、民谷栄一(北陸先端大)························································· 56

 

Nanoprobes by biopolymer

P41Two Kinds of Unique-Supramolocular Functions Based on Polyrotaxane Nano-Materials

Tooru Ooya and Nobuhiko Yui

Japan Advanced Institute of Science and Technology

○大谷亨、由井伸彦(北陸先端大)···························································· 57

 

P42Screening of Fullerene Binding Peptide by Phage Display Library and Combinatorial Chemistry

Takuya Oosugi, Yasutaka Morita and Eiichi Tamiya

Japan Advanced Institute of Science and Technology

○大杉卓也、森田資隆、民谷栄一(北陸先端大)············································· 58

 


P43Screening of STAT3 Binding Peptide by Phage Display Library

Arief Budi Witarto and Kenji Yokoyama

Japan Advanced Institute of Science and Technology

○アリフ・ブディ・ウィタルト、横山憲二(北陸先端大)···································· 59

 

P44Design of Fluorescence-based Sensor Peptides

Nobuaki Miyamoto and Kenji Yokoyama

Japan Advanced Institute of Science and Technology

宮本延明、○横山憲二(北陸先端大)························································· 60

 

P45Mechanical Unpacking System for Morphological Analysis of Chromosomes

Kazunori Otobe and Xin Lin

National Agriculture Research Center

○乙部和紀、林新(農業研究センター)······················································· 61