Table of Contents
Invited Oral Presentations
【S01】Probing
nanostructures on cell surfaces - the route to understanding cell membrane
organization and function
Prof.
Michael Edidin (Johns Hopkins Univ.)
マイケル・エディディン(ジョンズ・ホプキンス大学)········································ 1
【S02】Scanning
probe microscopic analyses of chromosome function and structure
Prof.
Tatsuo Ushiki (Niigata Univ.)
牛木辰男(新潟大学医学部)···································································· 4
【S03】Exploring the
restless nano-architecture of the cell membrane in living cells: Single
molecules' approach
Prof.
Akihiro Kusumi (Nagoya Univ.)
楠見明弘(名古屋大学院・生命理学)·························································· 7
【S04】Single-Molecule
Fluorometry for Nanobiotechnology
Dr.
Mitsuru Ishikawa (JRCAT)
石川満(アトムテクノロジー研究体)························································· 10
【S05】Nanobiotechnology
and bioelectronics
Prof.
Eiichi Tamiya (JAIST)
民谷栄一(北陸先端大)······································································· 12
Poster Presentations
Bioimaging by AFM
【P01】Development
and Application of Carbon Nanotube Probe to Atomic Force Microscopy
○Kunio Takeyasu, Yu-suke Masamura and Shige H. Yoshimura
Graduate School of Biostudies,
Kyoto Univ.
○竹安邦夫、正村祐介、吉村成弘(京大院生命科学工学)···································· 17
【P02】Nanostructural
Biology of Gene Function
○Yougo Maruyama, Shige H. Yoshimura and Kunio Takeyasu
Graduate School of Biostudies,
Kyoto Univ.
○円山由郷、吉村成弘、竹安邦夫(京大院生命科学工学)···································· 18
【P03】Analysis of
DNA-HMG2 Complex Using AFM
○Masato Saito, Yuji Murakami, Kenji Yokoyama, Shin-ichi Tate, and
Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○斉藤真人、村上裕二、横山憲二、楯真一、民谷栄一(北陸先端大)························· 19
【P04】Single
Molecule Analyses by TEM and AFM for Functional DNA Transaction Complexes
○Tsurimoto, T.1, Shiomi, Y.1, Usukura, J.2,
Masamura, K.3, Takeyasu, K.3, and Yoshikawa, H.1
1Nara Advanced
Institute of Science and Technology, 2Department of Anatomy, Nagoya
University, School of Medicine, 3Graduate School of Biostudies,
Kyoto University
○釣本敏樹、塩見泰史、臼倉治郎、正村祐介、竹安邦夫、吉川寛(奈良先端大、名古屋大学医学部、京大院生命科学工学)································································ 20
【P05】Analysis of
Higher-order Structure of Escherichia Coli
Genome by Using Atomic Force Microscopy
○Yu-suke Masamura1, Azam, Talukder Ali2, Akira
Ishihama2,and Kunio Takeyasu1
1Graduate School of
Biostudies, Kyoto University, 2Department of Molecular Genetics,
National Institute of Genetics
○正村祐介、Azam, Talukder Ali、石浜明、竹安邦夫(京大院生命科学工学、国立遺伝学研究所)···························································································· 21
【P06】Nanostructural
Biology of the Chromatin
○Shige H. Yoshimura and Kunio Takeyasu
Graduate School of Biostudies,
Kyoto Univ.
○吉村成弘、竹安邦夫(京大院生命科学工学)················································ 22
【P07】The Spiral
Structure of Human Chromosomes Observed by Atomic Force Microscopy (AFM)
○Kousuke Iwai, Osamu Hoshi, Jiro Hitomi, and Tatsuo Ushiki
Department of Anatomy and
Histology, Faculty of Medicine, Niigata University
○岩井孔良、星治、人見次郎、牛木辰男(新潟大医)········································· 23
【P08】Structural
Analysis of Chromosome Bands by Atomic Force Microscopy
○Osamu Hoshi, Kousuke Iwai, Jiro Hitomi, and Tatsuo Ushiki
Department of Anatomy and
Histology, Faculty of Medicine, Niigata University
○星治、岩井孔良、人見次郎、牛木辰男(新潟大医)········································· 24
【P09】AFM Manipulation
and Imaging of Human Chromosomes
○Ken-ichi Ogawa1, Masato Saito1, Yuji Murakami1,
Tatsuo Ushiki2, and Eiichi Tamiya1
1Japan Advanced
Institute of Science and Technology, 2Niigata University
○小川健一、斉藤真人、村上裕二、牛木辰男、民谷栄一(北陸先端大、新潟大医)·········· 25
【P10】High-resolution
Imaging of Chromosome Structure Using AFM
-Effect of Protein Extraction
on Chromosome Surface-
○S. Sugiyama1, X. Liu2, T. Yoshino1,
S. Hagiwara1, and T. Ohtani1
1National Food
Research Institute, 2Japan Atomic Energy Research Institute
○杉山滋、劉新旗、吉野智之、萩原昌司、大谷敏郎(食品総合研究所、日本原子力研究所)· 26
【P11】DC
Electric-field Induced DNA Stretching for AFM and Surface Chemical Study
○J. M. Kim1, T. Ohtani1 and H. Muramatsu2
1National Food
Research Institute, 2Seiko Instruments Inc.
○ キム・ジョンミン、大谷敏郎、村松宏(食品総合研究所、セイコーインスツルメンツ)··· 27
AFM Physics
【P12】Shadow Images
of Plasmid DNA by Field Electron Emission from a Point Source
○M. Hirade, K. Nakatani, S. Tate, T. Arai, and M. Tomitori
Japan Advanced Institute of
Science and Technology
○平出雅人、仲谷幸司、新井豊子、富取正彦(北陸先端大)·································· 28
【P13】STM Study of
a TiO2 (110) 1x2 Surface as an Oxide Substrate for Biological
Materials
○N. Edo, T. Arai and M. Tomitori
Japan Advanced Institute of Science and Technology
○江戸暢子、新井豊子、富取正彦(北陸先端大)·············································· 29
【P14】Microfabrication
of Silicon Tip for Atomic Force Microscope Application
○Takayuki Wakayama, Katsuhisa Shibuya, Nobuya Iwata, and Shoji Yamada
Center for New Materials, Japan
Advanced Institute of Science and Technology
○若山貴行、渋谷克久、岩田展也、山田省二(北陸先端大)·································· 30
【P15】Microfabrication
of Gallium Arsenide Tip for Atomic Force Microscope Application
○Nobuya Iwata, Takayuki Wakayama, Katsuhisa Shibuya, and Shoji Yamada
Center for New Materials, Japan
Advanced Institute of Science and Technology
○岩田展也、若山貴行、渋谷克久、山田省二(北陸先端大)·································· 31
Advanced Probe Microscope
【P16】Imaging Green
Fluorescent Protein-tagged Receptor Molecules in Cellular Membranes with
Near-field Scanning Optical Microscope (NSOM)
○Shinichiro Iwabuchi1, Jeeseong Hwang2 and
Michael Edidin1
1Department of
Biology, The Johns Hopkins University, 2Optical Technology Division,
National Institute of Standards and Technology
○岩渕紳一郎、Jeeseong Hwang、マイケル・エディディン(ジョンズ・ホプキンス大学、NIST)································································································· 32
【P17】Characterization
of Micro Optical Cantilever and Its Application to Biological Samples
○Hiroshi Muramatsu1, Takashi Niwa1, Susumu
Ichihara1, Yasuyuki Mitsuoka1, Jong-Min Kim2,
Patrick Degenaar3, Hiromi Fujita3, Mitsuyoshi Ueda4,
Eiichi Tamiya3
1Seiko Instruments, 2National
Food Research Institute, 3Japan Advanced Institute of Science and
Technology, 4Kyoto Univ.
○村松宏、新輪隆、市原進、光岡靖幸、キム・ジョンミン、パトリック・デガナー、藤田裕美、植田充美、民谷栄一(セイコーインスツルメンツ、食品総合研究所、北陸先端大、京大院工)·························································································· 33
【P18】SNOAM Imaging
of GFP Displayed on Yeast Cell Surfaces
○Hiromi Fujita, Mitsuyoshi Ueda*, Yasutaka Morita, and
Eiichi Tamiya
Japan Advanced Institute of
Science and Technology, Kyoto Univ. *
○藤田裕美、植田充美、森田資隆、民谷栄一(北陸先端大、京大院工)······················ 34
【P19】Near Field
Fluorescent Imaging of Neurite Growths from Patterned Neuron
○Patrick Degenaar, Laurent Griscom*, Yuji Murakami, Bruno
Le Pfioufle*, Hiroaki Fujita*, and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology, Tokyo University*
○パトリック・デガナー、Laurent Griscom、村上裕二、Bruno Le Pfioufle、藤田裕美、民谷栄一(北陸先端大、東大生研)·································································· 35
NMR, Fluorescence Microscope
and related works
【P20】NMR and CD
Analysis of the Short Basic Tail Following HMG-2B Domain in its Contribution to
DNA Binding
○Madoka Kanbayashi1, Jun-ichi Kurita1, Michiteru
Yoshida2, and Shin-ichi Tate1
1Center for New
Materials, Japan Advanced Institute of Science and Technology, 2Science
University of Tokyo
○上林円、栗田順一、吉田充輝、楯真一(北陸先端大新素材センター、東京理科大基礎工)· 36
【P21】High
Resolution NMR Structure Analysis of Two HMG-box Domains in Nucleosome Protein
HMG-2
○Kouji Nakatani1, Jun-ichi Kurita1, Masatsune
Kainosho2, Michiteru Yoshida3, and Shin-ichi Tate1
1Center for New
Materials, Japan Advanced Institute of Science and Technology,
2Tokyo Metropolitan
University, 3Science University of Tokyo
○仲谷幸司、栗田順一、甲斐荘正恒、吉田充輝、楯真一(北陸先端大新素材センター、東京都立大理、東京理科大基礎工)································································ 37
【P22】3D
Visualization of Gene Locations in a Barley Nucleus
○Kiichi Fukui
Department of Biotechnology,
Graduate School of Engineering, Osaka University
○福井希一(阪大院工)······································································· 38
【P23】Nuclear Volumes
Revealed by 3D Analyses in Plants
○Satoru Fujimoto, Mikako Ito and Kiichi Fukui
Department of Biotechnology,
Graduate School of Engineering, Osaka University
○藤本聡、伊藤美佳子、福井希一(阪大院工)················································ 39
【P24】Determination
of Genome and of Chromosomes Dimensions of Lotus Japonicus.
○Mikako Ito1, Junko Miyamoto2, Yumiko Mori1,
Satoru Fujimoto1, Toshiki Uchiumi2, Mikiko Abe2,
Akihiro Suzuki2, Satoshi Tabata3, and Kiichi Fukui1
1Osaka University, 2Kagoshima
University, 3Kazusa DNA Research Institute
○伊藤美佳子、宮本旬子、森裕美子、藤本聡、内海俊樹、阿部美紀子、鈴木章弘、田畑哲之、福井希一(阪大院工、鹿児島大、かずさDNA研究所)········································ 40
【P25】Isolation of
Human Metaphase Chromosomes by the Flow-sorting Method
○Takefumi Sone, Shouhei Kobayashi, Takeshi Ishihara, Yuki
Mitsubayashi, and Kiichi Fukui
Department of Biotechnology,
Graduate School of Engineering, Osaka University
○曽根岳史、小林昇平、石原武、三林有紀、福井希一(阪大院工)··························· 41
【P26】The
Development of a Digital Image Database of Chromosomes
○Junko Miyamoto1, Tamaki Hirose2, Yukio Akiyama3,
Toshiyuki Wako4, and Kiichi Fukui5
1Kagoshima Univ. 2Japan
Atomic Energy Research Institute, 3Japan Science and Technology
Corporation, Hokuriku National Agricultural Experiment Station, 4National
Institute of Agrobiological Resources, 5Graduate School of
Engineering, Osaka University
○宮本旬子、廣瀬玉紀、秋山征夫、若生俊行、福井希一(鹿児島大理、日本原子力研究所、農林水産省北陸農業試験場、農林水産省農業生物資源研究所、阪大院工)···················· 42
【P27】Proteome
Analysis of Human Chromosomes
○Shouhei Kobayashi1, Takefumi Sone1, Takeshi
Ishihara1, Yuki Mitsubayashi1, Toshifumi Takao2,
Shinsuke Fujiwara1, Masahiro Takagi1, and Kiichi Fukui1
1Department of
Biotechnology, Graduate School of Engineering, Osaka University,
2Institute for
Protein Research, Osaka University
○小林昇平、曽根岳史、石原武、三林有紀、高尾敏文、藤原伸介、高木昌宏、福井希一(阪大院工、阪大蛋白研)·········································································· 43
Microarray, biosensor and
related works
【P28】High
Throughput Single Cell PCR on a Microchamber Array
○Hidenori Nagai, Yuji Murakami and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○永井秀典、村上裕二、民谷栄一(北陸先端大)·············································· 44
【P29】In vitro Protein Synthesis on Microchamber Arrays
○Takeshi Kinpara1, Hideo Nakano2, Yuji Murakami1,
and Eiichi Tamiya1
1Japan Advanced
Institute of Science and Technology, 2Nagoya University
○金原健、中野秀雄、村上裕二、民谷栄一(北陸先端大)···································· 45
【P30】Microfabricated
Magnetic Particle as Bio-electrode on Array Chip
○Yuji Murakami, Akira Yamamura, Yong-Song Choi, and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○村上裕二、山村晃、チェ・ヨンソン、民谷栄一(北陸先端大)····························· 46
【P31】High-integrated
Biochip by Hydrophobic Interaction
○Yong-Song Choi, Do-Kyun Kim, Yuji Murakami, and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○チェ・ヨンソン、キム・ドキュン、村上裕二、民谷栄一(北陸先端大)···················· 47
【P32】Fabrication
of 2D Superlattices of Gold Nanoparticles
○Toshiharu Teranishi and Mikio Miyake
Japan Advanced Institute of
Science and Technology
○寺西利治、三宅幹夫(北陸先端大)························································· 48
【P33】Bioimaging of
Neural Cell Network on Microfabricated Chamber Array
○Yoshinori Akagi, Yuji Murakami and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○赤木良教、村上裕二、民谷栄一(北陸先端大)·············································· 49
【P34】Miniaturization
and Integration of SPR Immunosensors
○Masayasu Suzuki1, Fumihiko Ozawa1, Wakako
Sugimoto2, Takehiro Murashige2, and Shuji Aso2
1Toyama University, 2Kyushu
Institute of Technology
○鈴木正康、小沢文彦、杉本和歌子、村重剛弘、麻生秀司(富山大工、九州工業大情報工)· 50
【P35】Enzyme
Electrochemistry Mediated by SAM-based Redox Active Polymer
○Shu Taira, Eiichi Tamiya and Kenji Yokoyama
Japan Advanced Institute of
Science and Technology
○平修、民谷栄一、横山憲二(北陸先端大)·················································· 51
Microfluidics for biological
application
【P36】On-chip
Bypass Structure for Sample Segment Dilution
○Takashi Kanekiyo, Yuji Murakami and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○兼清崇、村上裕二、民谷栄一(北陸先端大)················································ 52
【P37】Electrochemical
Gene Detection with PCR Chip
○Masaaki Kobayashi1, Miyuki Oomura1, Takashi
Kusakawa2, Yasutaka Morita1, Yuji Murakami1,
Kenji Yokoyama1, and Eiichi Tamiya1
1Japan Advanced
Institute of Science and Technology, 2Hokuto Science Industry
○小林正昭、大村美由紀、草川貴史、森田資隆、村上裕二、横山憲二、民谷栄一(北陸先端大、北斗科学産業)············································································ 53
【P38】Allergy
Sensor with Mast Cell Cultivated on a Chip
○Yasutaka Matsubara, Yuji Murakami and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○松原泰孝、村上裕二、民谷栄一(北陸先端大)·············································· 54
【P39】Integrated
Microfluidic Devices for Chromosome Engineering
○Takahito Inoue and Hiroshi Yokoyama
Electrotechnical Laboratory
○井上貴仁、横山浩(経済産業省電子技術総合研究所)······································· 55
【P40】On Chip
Screening of Dioxin Binding Peptide
○Yasutaka Morita and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○森田資隆、民谷栄一(北陸先端大)························································· 56
Nanoprobes by biopolymer
【P41】Two Kinds of
Unique-Supramolocular Functions Based on Polyrotaxane Nano-Materials
○Tooru Ooya and Nobuhiko Yui
Japan Advanced Institute of
Science and Technology
○大谷亨、由井伸彦(北陸先端大)···························································· 57
【P42】Screening of
Fullerene Binding Peptide by Phage Display Library and Combinatorial Chemistry
○Takuya Oosugi, Yasutaka Morita and Eiichi Tamiya
Japan Advanced Institute of
Science and Technology
○大杉卓也、森田資隆、民谷栄一(北陸先端大)············································· 58
【P43】Screening of
STAT3 Binding Peptide by Phage Display Library
○Arief Budi Witarto and Kenji Yokoyama
Japan Advanced Institute of
Science and Technology
○アリフ・ブディ・ウィタルト、横山憲二(北陸先端大)···································· 59
【P44】Design of
Fluorescence-based Sensor Peptides
Nobuaki Miyamoto and ○Kenji
Yokoyama
Japan Advanced Institute of
Science and Technology
宮本延明、○横山憲二(北陸先端大)························································· 60
【P45】Mechanical
Unpacking System for Morphological Analysis of Chromosomes
○Kazunori Otobe and Xin Lin
National Agriculture Research
Center
○乙部和紀、林新(農業研究センター)······················································· 61